STUDIES IN RODENT POLIOMYELITIS

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Studies in Rodent Poliomyelitis

1. The murine virus may be grown in embryonic mouse brain-serum ultrafiltrate cultures. The virus fails to grow in embryonic chick tissue cultures or in fertilized egg preparations. 2. Some relationship can be demonstrated between the amount of nervous tissue and the infectivity of the culture. 3. Optimum titers of virus potency (10(-6)) can be obtained by adjusting the pH of the growing cultur...

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Studies in Rodent Poliomyelitis

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STUDIES IN RODENT POLIOMYELITIS I. FImTHER EXPERIMENTS WITH THE MURINE STI~AIN OF SK POLIOMYELITIS

In 1940, we reported the successful adaptation of the SK strain of human poliomyelitis virus from the rhesus monkey to white mice by way of intermediary passage through cotton rats (1). A close relationship between the adapted virus and monkey poliomyelitis virus was suggested by similarities in the symptomatology and pathology of the infection in monkeys and rodents as well as by the trend of ...

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Studies on the Purification of Poliomyelitis Virus

Results of experiments on the preparation of high molecular weight constituents from normal and poliomyelitis-infected medullae-cords are presented. Relatively large yields were obtained from glycerolated normal or infected tissues or from tissues that had been stored at -10 degrees C. for a few days. When the frozen tissues were stored for several weeks, the amount of sedimentable nitrogen iso...

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Attempts were made to "convert" highly virulent strains of the 3 immunologic types of poliomyelitis virus (Mahoney, Y-SK, and Leon) into avirulent variants. Tests involving intracerebral, intramuscular, or oral administration of virus to cynomolgus monkeys indicated that mere propagation in cultures of kidney tissue of cynomolgus monkeys had no effect on virulence when single or small numbers o...

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ژورنال

عنوان ژورنال: Journal of Experimental Medicine

سال: 1942

ISSN: 1540-9538,0022-1007

DOI: 10.1084/jem.76.1.31